Transcriptome changes associated with elongation of bovine conceptuses II: differentially expressed transcripts in the endometrium on day 17 after insemination

The objective was to characterize endometrial transcriptome on d 17 of gestation in dairy cows according conceptus length. Nonlactating Holstein (n = 48) were euthanized after AI and the uterine horn ipsilateral CL flushed with saline solution. Recovered conceptuses classified as small (1.2 6.9 cm; n 9), medium (10.5 16.0 or large (18.0 26.4 10). Samples intercaruncular endometrium dissected from caudal, intermediate, cranial portions pregnancy pooled for analyses. Total mRNA extracted tissue subjected analyses using Affymetrix Gene Chip Bovine array. Data normalized GCRMA method analyzed by robust regression Linear Models Microarray library within Bioconductor R. Transcripts P ≤ 0.05 adjustment false discovery rate fold change ≥1.5 considered differentially expressed. Functional conducted Ingenuity Pathway Analysis platform. Comparisons between endometria carrying vs. (LvsS), (LvsM), (MvsS) yielded a total 235, 21, 94 expressed transcripts, respectively. Top canonical pathways included antigen presentation pathway Th1/Th2 activation pathways, both LvsS MvsS. Interferon-α -γ identified activated upstream regulators, primarily based differently transcripts such IDO1, ISG20, WARS, LGALS9, IFI44, PSMB9 (LvsS MvsS). For LvsS, regulator revealed predicted FOXO1, IFN, NFACTC2, IL-12, IL-6, IL-18, whereas it depicted inhibition IL10RA ZBTB1. Changes these regulators associated downstream leukocytes, well quantity expansion T lymphocytes. Canonical comparison LvsM cell cycle G2/M DNA damage checkpoint regulation, control chromosomal replication. Moreover, tretinoin predicted, analysis same comparison. In conclusion, most compared counterparts involved modulation immune response.